https://ogma.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:40829 Wed 28 Sep 2022 10:43:45 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:44821 90% of the monolayers showed <1% ciliated EOECs). Occasionally (5–10%), re-differentiated monolayers with 11–27% EOECs with secondary cilia in a diffuse pattern were obtained. Additionally, nuclear progesterone receptor expression was found to be inhibited by simulated luteal phase hormone concentrations, and sperm binding to cilia was higher for re-differentiated EOEC monolayers exposed to estrogen–progesterone concentrations mimicking the follicular rather than luteal phase. Overall, a functional equine oviduct model was established with close morphological resemblance to in vivo oviduct epithelium.]]> Mon 24 Oct 2022 09:45:31 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:48585 0.05). After an early increase in membrane fluidity, a significant and cAMP-dependent increase in viable sperm with phosphatidylserine (PS), but not phosphatidylethanolamine (PE) exposure was noted. While the events observed partly resemble findings from the in vitro capacitation of sperm from other mammalian species, the lack of cholesterol removal appears to be an equine-specific phenomenon. This research will assist in the development of a defined medium for the capacitation of stallion sperm and will facilitate progress toward a functional IVF protocol for horse gametes.]]> Fri 21 Apr 2023 10:04:52 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:48463 Fri 17 Mar 2023 12:14:38 AEDT ]]>